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The D-Isocitric Acid test kit is for the specific and rapid measurement and analysis of D-isocitric acid in fruit juices.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of D-Isocitric Acid in foodstuffs
Principle:
(isocitrate dehydrogenase)
(1) D-Isocitric acid + NADP+ → 2-oxoglutarate + CO2 + NADPH + H+
(pH 9-10)
(2) D-Isocitric acid ester + H2O → D-isocitric acid + alcohol
(pH 9-10)
(3) D-Isocitric acid lactone + H2O → D-isocitric acid
Kit size: 100 assays (manual) / 1000 (microplate)
/ 1000 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.35 mg/L
Application examples:
Fruit juices, fruit products, soft drinks and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by EN, NEN, NF,
DIN, GOST, IFU and AIJN
Advantages
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- No wasted isocitrate dehydrogenase solution (stable suspension supplied)
- Very rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats